Specific 125I labeling of D1 (herbicide‐binding protein)

FEBS Letters

Inoue, Y.¹  Ikeuchi, M.¹ 

[1] Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-01, Japan

关键词: Iodination;    Photosystem II;    Protein D1;    Herbicide-binding protein;    Photosynthesis;    Mes;    2-(N-morpholino)ethanesulfonic acid;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    PS II;    photosystem II;   

DOI  :  10.1016/0014-5793(87)81300-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When ¹²⁵I⁻ was given as an artificial electron donor to non-O₂-evolving thylakoids of spinach, a 29 kDa polypeptide was specifically tagged by ¹²⁵I due to its photooxidation by PS II [(1985) Plant Cell Physiol. 26, 1093–1100]. We examined precisely the ¹²⁵I-labeling pattern in comparison with azido[¹⁴C]atrazine photoaffinity labeling of D1 and immunoblotting with anti-D1 and anti-D2, and found that D1 (herbicidebinding protein) of PS II reaction center complex is specifically tagged by ¹²⁵I in three different species of higher plants (spinach, pea and wheat) and a thermophilic cyanobacterium (Synechococcus vulcanus). It was suggested that D1 bears the photooxidation site or has a domain very close to the photooxidation site on the donor side of PS II, in addition to the well established binding site for Q_b and herbicides on the acceptor side of PS II.

【 授权许可】

Unknown