1H‐NMR analysis of the sugar structures of glycoproteins as their pyridylamino derivatives

FEBS Letters

Ikenaka, Tokuji²  Daiyasu, Hiromi²  Hase, Sumihiro²  Kyogoki, Yoshimasa¹  Koyama, Satoshi²  Kobayashu, Yuji¹ 

[1] Institute for Protein Research, Osaka University, Suita, Osaka 565, Japan;Department of Chemistry, Osaka University College of Science, Toyonaka, Osaka 560 Japan

关键词: Sugar structure;    Glycoprotein;    Oligosaccharide;    Pyridylamino derivative;    NMR Structural-reporter signal;    R;    GlcNAcβ1-4GlcNAc-PA;    M1;    Manβ1-4R;    M2;    Manα1-3Manβ1-4R;    M3;    Manα-1-6-(Manα1-3)Manβ1-4R;    M5;    Manα-1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4R;    M6;    Manα1-6(Manα1-3)Man-α1-6(Manα1-2Manα1-3)Manβ-4R;    M9;    Manα1-2Man-α6̄(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4R;    Man5GlcNAc4-PA;    Manα1-6-(Manα1-3)Manα1-6(GlcNA(β1-2Manα-1-3)(GlcNAcβ1-4)Manβ1-4R;    PA-biantennary sugar chain;    Ga1β1-4GlcNAcβ1-2Manα1-6(Ga1β1-4GlcNAcβ1-2Manα1-3)-Manβ1-4R;    PA-triantennary sugar chain;    Ga1β1-4GlcNAcβ1-2Manα1-6(Ga1β1-4GlcNAcβ1-2[Ga1β1-4GlcNAcβ1-4]Manα1-3)Manβ1-4R;    PA-;    pyridylamino;   

DOI  :  10.1016/0014-5793(86)81125-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ¹H-NMR spectra of a series of pyridylamino (PA-) derivatives of oligosaccharides were obtained and compared with those of the corresponding asparagine-linked sugar chains in order to elucidate the effect of the PA-group on the chemical shifts of structural-reporter signals. The effects were found to be localized within the two residues from the end group. Thus, the data for asparagine-linked chains in the literature are applicable to PA-derivatives, so the combination of pyridylamination and NMR measurements greatly reduces the time required for structure analysis of sugar chains of glycoproteins, because the isolation and purification of the chains as PA-derivatives are easy and efficient.

【 授权许可】

Unknown