Inhibition of phorbol ester stimulated superoxide production by 1‐oleoyl‐2‐acetyl‐sn‐glycerol (OAG); fact or artefact?

FEBS Letters

Hodson, H.F.¹  Thompson, N.T.²  Garland, L.G.²  Dawson, J.²  Bonser, R.W.² 

[1] Department of Medicinal Chemistry, The Wellcome Research Laboratories, Langley Court, Beckenham BR3 3BS, Kent, England;Department of Biochemistry, The Wellcome Research Laboratories, Langley Court, Beckenham BR3 3BS, Kent, England

关键词: Protein kinase C Phorbol ester Diacylglycerol (HL-60 cell) Superoxide;   

DOI  :  10.1016/0014-5793(86)81098-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

OAG-stimulated superoxide (O⁻ ₂) production by HL-60 granulocytes showed enantiomeric specificity but reached a maximum of only 5% of that produced by either phorbol myristate acetate (PMA) or phorbol dibutyrate (PDBu). At 10–100 μM, OAG displaced specifically-bound [³H]PDBu from intact HL-60 cells by only 25%, suggesting limited cell penetration. OAG (10–100 μM) also inhibited PDBu-stimulated O₂ production by 25%; this inhibition was enantiomerically specific. However, at a lower concentration (3 μM), both enantiomers of OAG fully blocked O⁻ ₂ production stimulated by PMA (0.5 μM). This inhibition is probably artefactual, due to the hydrophobic PMA physically associating with OAG in the extracellular fluid.

【 授权许可】

Unknown