The growth of ordered two‐dimensional sheets of 70 S ribosomes from Bacillus stearothermophilus

FEBS Letters

Gewitz, H.S.²  Arad, T.¹  Yonath, A.¹  Piefke, J.²  Wittmann, H.G.² 

[1] Dept of Structural Chemistry, Weizmann Institute of Science, Rehovot, Israel;Max-Planck-Institut für Molekulare Genetik, D-1000 Berlin(Dahlem, Germany

关键词: Ribosome Two-dimensional sheet 70 S ribosomal particle (Bacillus stearothermophilus);   

DOI  :  10.1016/0014-5793(86)81092-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Well ordered two-dimensional sheets of intact 70 S ribosomes from Bacillus stearothermophilus have been obtained in vitro using salt-alcohol mixtures. These sheets consist of relatively small unit cells with dimensions of 200 ± 20 Å and 400 ± 30 Å. Diffraction patterns of electron micrographs of these sheets stained with uranyl acetate contain features to 42 Å resolution.

【 授权许可】

Unknown