Immunological evidence for the presence of the D1 and D2 proteins in PS II cores of higher plants

FEBS Letters

Dyer, T.A.³  Nixon, P.J.¹  Barber, J.¹  Hunter, C.N.² 

[1] AFRC Photosynthesis Research Group, Imperial College of Science and Technology, London SW7 2BB England;Department of Pure and Applied Biology, Imperial College of Science and Technology, London SW7 2BB England;Plant Breeding Institute, Cambridge CB2 2LQ, England

关键词: Photosynthesis Photosystem II Antibody Gene fusion D1 protein D2 protein;   

DOI  :  10.1016/0014-5793(86)81088-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The psb A and psb D genes isolated from Poa annua and Triticum aestivum, respectively, have been expressed in Escherichia coli as β-galactosidase fusion proteins and used to raise antibodies. The antibodies to the psb D gene product cross-reacted with a lysine containing polypeptide with an apparent molecular mass of 31 kDa which was present in a PS II core preparation. In contrast, the antibody to thepsb A gene product recognised predominantly, a 34 kDa protein in the PS II core which was insensitive to treatments with a lysine specific protease. It is concluded that the latter polypeptide is the lysine-free D1 protein and that the antibody raised to the psb D gene product monospecifically reacted with a component presumed to be the D2 protein. It is therefore suggested that the product of the psb D gene, as well as that of the psb A gene, is a component of the PS2 core complex of higher plants.

【 授权许可】

Unknown