Preferential binding of collagenase to α2‐macroglobulin in the presence of the tissue inhibitor of metalloproteinases

FEBS Letters

Cawston, Timothy E.¹  Mercer, Elizabeth¹ 

[1] Rheumatology Research Unit, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, England

关键词: Collagenase α2-Macroglobulin Cleavage site Connective tissue breakdown Enzyme-inhibitor complex TIMP;   

DOI  :  10.1016/0014-5793(86)81074-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding of collagenase to both α2-niacroglobulin and the tissue inhibitor of metalloproteinases was studied using purified materials. Collagenase bound preferentially to α₂-macroglobulin although no transfer of collagenase to α₂-macroglobulin occurred if the enzyme was first mixed with the tissue inhibitor of metalloproteinases. The sequences of amino acids in both inhibitors likely to be responsible for the binding of collagenase are discussed and compared to the cleavage site in the collagen molecule.

【 授权许可】

Unknown