Lack of adenosine deaminase deficiency in the mutant mouse wasted

FEBS Letters

Geiger, J.D.²  Nagy, J.I.¹ 

[1]Department of Physiology, University of Manitoba Faculty of Medicine, 770 Bannatyne Avenue, Winnipeg, Manitoba R3E OW3, Canada

[2]Department of Pharmacology, University of Manitoba Faculty of Medicine, 770 Bannatyne Avenue, Winnipeg, Manitoba R3E OW3, Canada

关键词: Adenosine deaminase Adenosine Animal model Severe combined immunodeficiency disease;   

DOI  :  10.1016/0014-5793(86)81063-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possibility that the mutant mouse wasted (wst/wst) may serve as an animal model for studies of severe combined immunodeficiency disease (SCID) and the role of adenosine deaminase (ADA, EC 3.5.4.4) in adenosine metabolism were investigated. The specific activity of ADA in wst/wst compared with control mice was significantly lower by 26% in thymus, but significantly higher by 18% in spleen and 32% in cerebellum. V _max values of ADA in spleens were 4356 higher in wst/wst mice and no changes were observed in K _m values. In contrast, the V _max of ADA was unchanged in erythrocytes from wst/wst mice, but the K _m for adenosine was significantly elevated. Thus, based on ADA measurements alone, it may be premature to consider wst/wst mice as a model for ADA deficiency and SCID in humans.

【 授权许可】

Unknown