Turnover of rat liver ornithine transcarbamylase

FEBS Letters

Wallace, Ruth¹  Knecht, Erwin¹  Grisolía, Santiago¹ 

[1] Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Amadeo de Saboya 4, 46010 Valencia, Spain

关键词: Protein turnover Mitochondria Lysosome Ornithine carbamoyltransferase;    δ-PALO;    δ-N-(phosphonoacetyl)-L-ornithine;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    t;    half-lives;    k d;    rate constant of degradation;   

DOI  :  10.1016/0014-5793(86)81062-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The relative half-life of ornithine transcarbamylase from rat liver has been determined using the double isotope technique and affinity chromatography. The calculated half-life (6–9 days) is similar to that of mitochondria and of the other mitochondrial enzyme of the urea cycle, carbamoyl-phosphate synthase. Therefore, both mitochondrial urea cycle enzymes are most probably degraded mainly via the lysosomal (autophagic) pathway of mitochondrial protein degradation.

【 授权许可】

Unknown