Extrachromosomal DNA from a dicot plant Vigna radiata

FEBS Letters

Bhattacharyya, Nisan¹  Roy, Pranab¹ 

[1] Department of Biochemistry, Bose Institute (Centenary Building), P-1/12, CIT Scheme VII M, Calcutta 700 054, India

关键词: extrachromosomal DNA Polydisperse size Repetitive sequence;    EC DNA;    extrachromosomal DNA;    ccc;    covalently closed circular;    HAP;    hydroxyapatite;    bp;    base pair;    EtBr;    ethidium bromide;    MS medium;    Murashige and Skoog's medium;   

DOI  :  10.1016/0014-5793(86)81054-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Extrachromosomal DNA was isolated from germinated mung bean as well as from its callus culture. Methods followed for the isolation of cccDNA gave a heterogeneous mixture of DNA from 4.2 kb to 140 bp in size. Hybridization with a repetitive DNA probe from mung bean chromosomal DNA showed extensive dispersion, similar in chromosomal and extrachromosomal locations. An electron micrograph of purified extrachromosomal DNA revealed some circular DNA molecules with hanging tails.

【 授权许可】

Unknown