Human chorionic gonadotropin activates the inositol 1,4,5‐trisphosphate‐Ca2+ intracellular signalling system in bovine luteal cells

FEBS Letters

Farese, Robert V.¹  Davis, John S.¹  Weakland, Laura L.¹  West, Leigh A.¹ 

[1] James A. Haley Veterans Administration Hospital and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, FL 33612, USA

关键词: Ca2+Gonadotropin Inositol phospholipid Inositol phosphate (Corpus luteum);    hCG;    human chorionic gonadotropin;    [Ca2+]i;    cytosolic free calcium concentration;    quin 2/AM;    quin 2 tetraacetoxymethyl ester;    IP3;    inositol trisphosphate;    IP2;    inositol bisphosphate;    IP;    inositol phosphate;    PI;    phosphatidylinositol;    PIP;    phosphatidylinositol 4-phosphate;    PlP2;    phosphatidylinositol 4;    5-biphosphate;    DAG;    1;    2-diacylglycerol;   

DOI  :  10.1016/0014-5793(86)81034-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human chorionic gonadotropin, hCG, a hormone which increases intracellular cAMP, provoked rapid (30 s) and sustained (up to 30 min) increases in the levels of inositol mono-, bis- and trisphosphates (IP, IP₂ and IP₃, respectively) in bovine luteal cells. LiCl (10 mM) enhanced inositol phosphate accumulation in response to hCG. Concentration-dependent increases in inositol phosphates, cAMP and progesterone accumulation were observed in hCG-treated luteal cells. hCG also induced rapid and concentration-dependent increases in cytosolic free Ca²⁺ as measured by quin 2 fluorescence. These findings demonstrate that hCG stimulates the phospholipase C-IP₃ and diacylglycerol ‘second messenger’ system in the bovine corpus luteum.

【 授权许可】

Unknown