Evidence for the repetitive domain structure of pig calpastatin as demonstrated by cloning of complementary DNA

FEBS Letters

Zenita, Koichi²  Takano, Emiko²  Hatanaka, Masakazu²  Marti, Thomas³  Kannagi, Reiji²  Sakihama, Toshiko³  Maki, Masatoshi¹  Mori, Hirotaka²  Murachi, Takashi²  Titani, Koiti³ 

[1] Institute for Virus Research Kyoto University, Kyoto 606, Japan;Department of Clinical Science and Laboratory Medicine, Faculty of Medicine, Kyoto 606, Japan;Department of Biochemistry, University of Washington, Seattle, WA 98195, USA

关键词: cDNA Calpastatin Domain structure;   

DOI  :  10.1016/0014-5793(86)81017-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A clone of complementary DNA (cDNA) coding for pig heart calpastatin was isolated using synthetic oligonucleotide probes. The amino acid sequence deduced from the nucleotide sequence revealed the occurrence of a repetitive sequence at the interval of 140 amino acids, substantiating the multidomain structure of calpastatin. A portion of the sequence of 251 amino acid residues predicted for pig heart calpastatin (107 kDa) was found to be identical with that of a peptide fragment derived from pig erythrocyte calpastatin (68 kDa) and sequenced by Edman degradation.

【 授权许可】

Unknown