Immunoactive chimeric ST‐LT enterotoxins of Escherichia coli generated by in vitro gene fusion

FEBS Letters

Sanchez, J.³  Grundström, T.¹  Uhlin, B.E.²  Hirst, T.R.³  Holmgren, J.³ 

[1] Unit of Applied Cell and Molecular Biology, University of Umeå, S-901 87 Umeå, Sweden;Department of Microbiology, University of Umeå, S-901 87 Umeå, Sweden;Department of Medical Microbiology, University of Göteborg, Guldhedsgatan 10, S-413 46 Göteborg, Germany

关键词: Gastrointestinal disease Hybrid protein Bacterial vaccine Immunoenzyme technique Synthetic gene;   

DOI  :  10.1016/0014-5793(86)81016-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two different lengths of the gene encoding Escherichia coli heat-stable toxin (STa) were fused to the carboxy end of the gene coding for the E. coli heat-labile toxin A-subunit (LT_A). The hybrid genes directed expression of chimeric LT_A-STa proteins. Association of these chimeras with native heat-labile toxin B-subunit (LT_B) resulted in protein complexes that bound to GM₁ ganglioside and thereby could be assayed in a GM₁ ELISA. The complexes reacted with monoclonal antibodies against either LT_A, LT_B or STa indicating that the STa and LT epitopes remained immunologically intact after fusion. Genetically constructed chimeric proteins exhibiting LT and STa antigens on the same molecule may represent a promising approach to development of broadly protective immunoprophylactic agents and/or useful immunodiagnostic reagents for diarrhoeal diseases caused by enterotoxinogenic E. coli.

【 授权许可】

Unknown