Primary structure of elongation factor 1β from Artemia

FEBS Letters

Maassen, J.A.¹  Möller, W.¹  Maessen, G.D.F.¹  Amons, R.¹ 

[1] Dept. Medical Biochemistry, Sylvius Laboratories, PO Box 9503, 2300 RA Leiden, The Netherlands

关键词: (Artemia salina) Protein synthesis Elongation factor 1β Guanine nucleotide exchange protein Nucleotide sequence cDNA cloning γgt 11 phage library;   

DOI  :  10.1016/0014-5793(86)81536-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

cDNA complementary to mRNA coding for the elongation factor EF-1β has been cloned. A γgt 11 cDNA library has been screened with an antiserum against EF-1β which exchanges GDP bound to EF-1α with exogenous GTP during protein synthesis. The derived amino acid sequence corresponds to 208 amino acids including the N-terminal methionine which is absent in the mature protein. About sixty percent of the protein was sequenced by direct protein sequence analysis. Comparison of Artemis salina EF-1β with Escherichia coli EF-Ts shows no evident homology.

【 授权许可】

Unknown