Gold sodium thiomalate activates latent human leukocyte collagenase

FEBS Letters

Lindy, Seppo¹  Sorsa, Timo¹  Suomalainen, Kimmo¹  Turto, Heikki¹ 

[1] Department of Medical Chemistry, University of Helsinki, Siltavuorenpenger 10, SF-00170 Helsinki, Finland

关键词: Latent human leukocyte collagenase Gold sodium thiomalate Activation Ultraviolet difference absorbance technique;   

DOI  :  10.1016/0014-5793(86)81523-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gold sodium thiomalate, a drug used widely in the therapy of rheumatoid arthritis, was found to be an activator of latent human polymorphonuclear leukocyte collagenase. The activation was demonstrated by two distinct and independent collagenase assays: (i) by recording with a spectrophotometer at 227 nm the enzyme-induced increase in ultraviolet difference absorbance of native type I collagen connected to the cleavage of collagen at 37°C[(1986) Eur. J. Biochem. 156, 1-4] and (ii) by SDS-polyacrylamide gel electrophoresis analysis of formation of specific products of collagen resulting from collagenase cleavage at 25°C. Activation of latent collagenase by gold sodium thiomalate appeared to be of the same magnitude as by the known activator phenylmercuric chloride.

【 授权许可】

Unknown