Induction by recombinant human granulocyte colony‐stimulating factor of differentiation of mouse myeloid leukemic M1 cells

FEBS Letters

Tomida, Mikio¹  Okabe, Tetsuro¹  Yamamoto-Yamaguchi, Yuri¹  Hozumi, Motoo¹  Takaku, Fumimaro¹ 

[1] Department of Chemotherapy, Saitama Cancer Center Research Institute, Ina-machi, Kita-adachi-gun, Saitama-ken 362, Japan

关键词: Recombinant granulocyte colony-stimulating factor;    (Myeloid leukemic cell);    Differentiation;    Macrophage;    Differentiation-inducing factor;    G-CSF;    granulocyte colony-stimulating factor;    D-factor;    differentiation-inducing factor;    cDNA;    complementary DNA;   

DOI  :  10.1016/0014-5793(86)81503-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of recombinant human granulocyte colony-stimulating factor (G-CSF) on induction of differentiation of mouse myeloid leukemic M1 cells was examined. Purified G-CSF caused dose-dependent induction of phagocytic activity and lysozyme activity in M1 cells. Its half-maximally effective concentration was 10 ng/ml. On treatment of M1 cells with G-CSF (100 ng/ml) for 4 days, 30–50% of the cells differentiated morphologically into macrophage cells; 30–40% of the cells were blast cells and 20–30% of the cells were forms intermediate between blastic cells and mature macrophages.

【 授权许可】

Unknown