3′‐Hydroxymethyl 2′‐deoxynucleoside 5′‐triphosphates are inhibitors highly specific for reverse transcriptase

FEBS Letters

Kutateladze, Tamara V.¹  Kritzyn, Anatoli M.¹  Florentjev, Vladimir L.¹  Beabealashvilli, Robert Sh.¹  Chidgeavadze, Zurab G.¹  Kavsan, Vadim M.¹ 

[1] Institute of Physiology, Georgian SSR Academy of Sciences, Tbilisi 380060, USSR

关键词: DNA polymerase;    Terminal transferase;    Chain terminator;    Nucleotide analog;   

DOI  :  10.1016/0014-5793(86)81489-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

dNTP(3′-OCH₃), a 3′-O-methyl derivative of dNTP, is a chain terminator substrate for DNA synthesis catalyzed by AMV reverse transriptase. The enzyme seems to be the only DNA polymerase susceptible to the inhibitor while all the other DNA polymerases tested are fully resistant to the nucleotide analog. The resistant polymerases are: E. coli DNA polymerase I, Klenow's fragment of DNA polymerase I, phage T₄ DNA polymerase, calf thymus DNA polymerase α, rat liver DNA polymerase β and calf thymus terminal deoxyribonucleotidyl transferase.

【 授权许可】

Unknown