Purification of a 32.5 kDa monomeric sulfotransferase from rat liver with activity for bile acids and phenolic steroids

FEBS Letters

Kaplowitz, Neil¹  Stolz, Andrew¹  Takikawa, Hajime¹ 

[1] Liver Research Laboratory, Medical and Research Services, Wadsworth VA Medical Center and UCLA School of Medicine, Los Angeles, CA 90073, USA

关键词: Sulfotransferase;    Bile acid;    Phenolic steroid;    (Rat liver cytosol);    Protein purification;   

DOI  :  10.1016/0014-5793(86)81487-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Both bile acid and phenolic steroid sulfotransferase activities in rat liver cytosol have previously been identified in fractions corresponding to apparent molecular masses of 60–70 and 30–35 kDa. We purified the latter activity corresponding to a monomeric protein. Activity for bile acids and phenolic steroids co-eluted on sequential chromatography on Sephadex G-75 sf, Affigel blue, chromatofocusing and hydroxyapatite. The protein was homogeneous on SDS-PAGE (32.5 kDa).

【 授权许可】

Unknown