Possible incorporation of phosphoserine into globin readthrough protein via bovine opal suppressor phosphoseryl‐tRNA

FEBS Letters

Mizutani, Takaharu¹  Tachibana, Yoshio¹ 

[1] Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467, Japan

关键词: Phosphoserine;    Phosphoprotein;    Opal suppressor;    Seryl-tRNA;    Readthrough protein;    (Rabbit reticulocyte lysate);   

DOI  :  10.1016/0014-5793(86)80032-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Suppressor [³²P]phosphoseryl-tRNA, prepared using bovine seryl-tRNA synthetase and ATP:seryl-tRNA phosphotransferase, was mixed with rabbit reticulocyte lysates containing endogenous hemoglobin mRNA having the termination codon UGA (opal). The Chromatographic pattern of the lysate on Sephacryl S-200 showed that the radioactivity of [³²P]Pjphosphate in the hot trichloroacetic acid-precipitate (phosphoprotein) was eluted at the position between mature hemoglobin and globin subunits. The phosphoprotein, obtained by chromatography on S-200, moved to the position corresponding to that of globin readthrough protein on SDS-PAGE. The analyses of the hydrolyzate of the phosphoprotein showed the presence of phosphoserine in the protein. These results suggest that animal opal suppressor tRNA functions in vitro to transfer phosphoserine to the position of the termination codon UGA (opal) on mRNA.

【 授权许可】

Unknown