Purification and characterization of low potential c cytochromes from Desulfovibrio desulfuricans membranes

FEBS Letters

Pope, Daniel H.¹  Salerno, J.C.¹  Kramer, Jeffrey F.¹ 

[1] Biophysics Group and Biology Department, Rensselaer Polytechnic Institute, Troy, NY 12181, USA

关键词: Cytochrome;    Membrane protein;    Electron transfer;    Energy transduction (Desulfovibrio desulfuricans) Sulfate reduction;    DEAE;    diethylaminoethyl;    PAGE;    polyacrylamide gel electrophoresis;    PMS;    phenazine methosulfate;    PES;    phenazine ethosulfate;   

DOI  :  10.1016/0014-5793(86)81360-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Desulfovibrio desulfuricans grown in a lactate-sulfate medium produces, in addition to soluble cytochromes, c-type cytochromes which appear to be integral membrane proteins. Two cytochromes can be separated, an abundant 15 kDa cytochrome and a 22 kDa cytochrome. Both have optical spectra characteristics of c-type cytochromes. The 15 kDa cytochrome shows two n = 1 components in potentiometric redox titrations with midpoint potentials at −130 and −270 mV in the membrane; both were slightly lower in detergent-solubilized preparations. We suggest a designation of cytochrome cc _m for this species. Its properties suggest a function as a transmembrane electron carrier between hydrogen and sulfate.

【 授权许可】

Unknown