Induction of cell surface insulin antigenicity in fibroblasts transfected with islet genomic DNA

FEBS Letters

Nesher, Rafael²  Klein, Benjamin Y.¹  Cerasi, Erol² 

[1] The Lautenberg Center for General and Tumor Immunology, Hebrew University Medical School, POB 1172, Jerusalem 91010 Israel;Department of Endocrinology and Metabolism, Hadassah Medical Center, POB 12000, Jerusalem 91120, Israel

关键词: Fluorescent marker Cell surface Insulin Transient expression (L-cell) genomic DNA;   

DOI  :  10.1016/0014-5793(86)80925-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ltk⁻cells were transfected with total genomic DNA obtained from rat islets or from insulinoma cells mixed with DNA tagged with fluorescent dye. Flyorescence-activated cell sorter (FACS) was used for initial selection of successful transfectants, monitoring fluorescent DNA incorporated into the cell. For subsequent selections the cells were treated with anti-insulin antiserum labeled with fluorescent dye and selected by FACS. B cell DNA, tiut not DNA from murine leukemic cells, induced the appearance of cell surface insulin antigenicity in fibroblasts. This phenotypic expression was transient. Together with our previous demonstration of the induction of insulin secreation in Ltk⁻ cells [(1986) Biochem. Biophys. Res. Commun. 136, 638-644], these results indicate that B cell specific characteristics can be transfected to non-endocrine cells by genomic DNA transfection.

【 授权许可】

Unknown