In vitro synthesis of catalase protein in sweet potato root microbodies

FEBS Letters

Sakajo, Shigeru¹  Asahi, Tadashi¹ 

[1] Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University, Chikusa, Nagoya 464, Japan

关键词: Catalase Microbody Glyoxysome Peroxisome (Sweet potato);    IgG;    immunoglobulin G;    PAGE;    polyacrylamide gel electrophoresis;    poly(A)+;    polyadenylated;   

DOI  :  10.1016/0014-5793(86)80924-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Poly(A)⁺ RNA prepared from sweet potato root tissue was translated in a wheat germ in vitro translation system. A translation product was immunoprecipitated with anti-sweet potato catalase immunoglobulin G. The product was identical to the subunit of the catalase with respect to the mobility on an SDS-polyacryl-amide gel and the pattern of the peptide map, indicating that the catalase protein is synthesised in vitro in the same size as the mature subunit. No amino acids were released from the purified enzyme protein by Edman degradation, suggesting the occurrence of a minor modification in the N-terminal part of the protein during the enzyme formation.

【 授权许可】

Unknown