Characterization of the distribution of potential short restriction fragments in nucleic acid sequence databases

FEBS Letters

Tung, Chang-Shung¹  Burks, Christian¹ 

[1] Theoretical Biology and Biophysics Group, MS K710, Los Alamos National Laboratory, University of California, Los Alamos, NM 87545, USA

关键词: Nucleic acid sequence database Oligonucleotide synthesis Pattern recognition Restriction endonuclease;   

DOI  :  10.1016/0014-5793(86)80916-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have searched the GenBank nucleic acid sequence database for potential short restriction fragments. All possible oligonucleotides up to length five are found at least once flanked by known restriction recognition patterns. Thus, searches in the database for a specific sequence corresponding to a desired oligonucleotide would often point to one or more sources of short, retrievable fragments containing that sequence. These results underscore the potential of nucleic acid sequence databases in planning experiments.

【 授权许可】

Unknown