Inhibition of the mitochondrial tricarboxylate carrier by arginine‐specific reagents

FEBS Letters

Zaki, L.¹  Zara, V.²  Prezioso, G.²  Stipani, I.²  Palmieri, F.² 

[1] Max-Planck-Institut für Biophysik, 6000 Frankfurt am Main 71, FRG;Department of Pharmaco-Biology, Laboratory of Biochemistry, University of Bari, CNR Unit for the Study of Mitochondria and Bioenergetics, 70126 Bari, Italy

关键词: Tricarboxylate carrier Arginine-specific reagent Mitochondria Membrane transport (Rat liver);   

DOI  :  10.1016/0014-5793(86)80913-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of arginine-specific reagents on the activity of the partially purified and reconstituted tricarboxylate carrier of the inner mitochondrial membrane has been studied. It has been found that 1,2-cyclohexanedione, 2,3-butanedione, phenylglyoxal and phenylglyoxal derivatives inhibit the reconstituted citrate/citrate exchange activity. The inhibitory potency of the phenylglyoxal derivatives increases with increasing hydrophilic character of the molecule. Citrate protects the tricarboxylate carrier against inactivation caused by the arginine-specific reagents. Other tricarboxylates, which are not substrates of the carrier, have no protective effect. The results indicate that at least one essential arginine residue is located at the substrate-binding site of the tricarboxylate carrier and that the vicinity of the essential arginine(s) has a hydrophilic character.

【 授权许可】

Unknown