Regulation of insulin secretion by cAMP in rat islets of Langerhans permeabilised by high‐voltage discharge

FEBS Letters

Fyles, Julie M.¹  Howell, Simon L.¹  Jones, Peter M.¹ 

[1] Department of Physiology, King's College London (KQC), Campden Hill Road, Kensington, London W8 7 AH, England

关键词: Insulin secretion (Islets of Langerhans) Electrical permeabilization cyclic AMP Forskolin Isobutylmethylxanthine;   

DOI  :  10.1016/0014-5793(86)80898-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Adenosine 3',5-cyclic monophosphate (cAMP) was shown to stimulate insulin secretion from electrically permeabilised islets of Langerhans incubated in Ca²⁺/EGTA buffers. cAMP-induced insulin secretion occurred in the presence of either sub-stimulatory (50 nM) or stimulatory (> 100 nM) concentrations of Ca²⁺. Similar effects on secretion were obtained in response to 8-bromo-cAMP (8-Br-cAMP) or the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine. Forskolin (0.2–20μM) increased adenylate cyclase activity and enhanced insulin secretion from the permeabilised islets. These results suggest that, in electrically permeabilised islets, cAMP-induced insulin secretion is not dependent on changes in cytosolic Ca²⁺.

【 授权许可】

Unknown