Dolichol alters dynamic and static properties of mouse synaptosomal plasma membranes

FEBS Letters

Strong, R.²  Gorka, C.²  Williamson, L.S.²  Schroeder, F.³  Sun, G.Y.⁴  Wood, W.G.²  Sun, A.Y.¹ 

[1] University School of Medicine, St. Louis, MO 63125, USA;Departments of Biochemistry University School of Medicine, University of Missouri, Columbia, MO 65212, USA;Department of Pharmacology University School of Medicine, University of Missouri, Columbia, MO 65212, USA;Geriatric Research, Education and Clinical Center, VA Medical Center and Department of Internal Medicine, St. Louis, USA

关键词: Dolichol;    Fluorometry;    ESR;    Plasma membrane;    Isoprenolog;   

DOI  :  10.1016/0014-5793(86)80858-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dolichols are isoprenologues that are found in almost all tissues and whose biochemical function, aside from dolichol phosphate precursors, is not known. In addition, an understanding of the organizational and dynamic properties of dolichols in biological membranes has not been forthcoming. The purpose of the experiments reported here were to examine the effects of dolichol on the physical properties of mouse synaptic plasma membranes (SPM). Differential polarized phase fluorometry indicated that dolichol both fluidized and regidified SPM. Membrane areas detected by diphenylhexatriene and trans-parinaric acid were selectively fluidized and rigidified, respectively. It also was found that the spin label, 5-doxyl stearic acid indicated that dolichol reduced membrane fluidity. These results report for the first time a stuctural effect of dolichol on a biological membrane.

【 授权许可】

Unknown