Insulin stimulation of glucose uptake and the transmembrane potential of muscle cells in culture

FEBS Letters

Walker, Denise¹  Klip, Amira¹  Ramlal, Toolsie¹ 

[1] The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada

关键词: Hexose uptake;    Membrane potential;    (1–6 cell);    Insulin;    Second messenger;   

DOI  :  10.1016/0014-5793(86)80855-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The membrane potential of L6 muscle cells was measured with the fluorescent dye bis-oxonol. Hyperpolarizations of up to 15 mV were caused by gramicidin (in N-methyl-D-glucamine⁺ medium), or by monensin or ionomycin. Depolarization was achieved with gramicidin (in Na⁺ medium), or with K⁺. Insulin did not change the resting membrane potential of − 70 mV, yet it effectively stimulated 2-deoxy-D-glucose uptake. Conditions that hyperpolarize the cells did not alter the basal rate of hexose uptake. Moreover, insulin was still capable of stimulating hexose uptake in depolarized cells. It is concluded that modulation of the membrane potential is probably not a signalling event in insulin stimulaton of hexose uptake.

【 授权许可】

Unknown