31P‐NMR studies of Mycoplasma gallisepticum cells using a continuous perfusion technique

FEBS Letters

Rottem, Shlomo²  Egan, William¹  Barile, Michael² 

[1] Biophysics Laboratory, Center for Drugs and Biologies, Food and Drug Administration, 8800 Rockville Pike, Bethesda, MD 20892, USA;Mycoplasma Laboratory, Center for Drugs and Biologies, Food and Drug Administration, 8800 Rockville Pike, Bethesda, MD 20892, USA

关键词: 31P-NMR (Mycoplasma gallisepticum) Metabolism Glycolysis;   

DOI  :  10.1016/0014-5793(86)80846-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

³¹P-NMR studies of Mycoplasma gallisepticum cells have been carried out using a continuous perfusion technique; these are the first such studies with this organism. Using this technique, glucose metabolism was monitored in the intact organisms, and cell extracts were prepared to identify the intermediates. Under glycolytic conditions, high levels of fructose-1,6-diphosphate were observed, indicating that this sugar may play a key role in the regulation of metabolism. The level of phosphoenolpyruvate was low under normal glycolytic conditions, and did not increase during starvation. From the position of the internal inorganic phosphate peak, the intracellular pH was estimated. The cells were found to maintain an intracellular pH of ~ 7.1 over an investigated external pH range of 6.6–8.6.

【 授权许可】

Unknown