One‐ and two‐dimensional 90.5‐MHz 13C‐NMR spectroscopy of the N‐linked triantennary oligosaccharide units of calf fetuin

FEBS Letters

Berman, Elisha¹  Bendel, Peter¹ 

[1] Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot 76100 and School of Chemistry, Tel Aviv University, Ramat Aviv, Israel

关键词: Triantenary oligosaccharide two-dimensional NMR spectroscopy13C-NMR Fetuin Structural microheterogeneity (Calf);    2D;    two-dimensional;    Man;    D-mannopyranose;    Gal;    D-galactopyranose;    GlcNAc;    N-acetylglucosamine;    NeuAc;    N-acetylneuraminic acid;   

DOI  :  10.1016/0014-5793(86)80823-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Complete assignments of all anomeric resonances in the proton and carbon spectra of the N-linked oligosaccharide units of fetuin were made using one- and two-dimensional NMR spectroscopy. We are able to confirm the presence of microheterogeneity in the N-acetylneuraminic acid linkages to the galactose residues and the presence of a unique triantennary structure which carries a side chain: NeuAcα(1–3)Galβ(1–3) GlcNacβ(1–4)-. Anomeric carbon chemical shifts changes resulting from long-range conformational effects were observed.

【 授权许可】

Unknown