Redox studies of the tetraheme cytochrome c 3 isolated from the propionate‐oxidizing, sulfate‐reducing bacterium Desulfobulbus elongatus

FEBS Letters

Legall, Jean²  Samain, Eric¹  Albagnac, Guy¹ 

[1] Institut National de la Recherche Agronomique, Station de Technologie Alimentaire, BP 39, F59651 Villeneuve d'Ascq, France;Department of Biochemistry, University of Georgia, Athens, GA 30602, USA

关键词: Cytochrome c3(Desulfobulbus) Redox potentiometry Succinate pathway;   

DOI  :  10.1016/0014-5793(86)80821-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A tetraheme cytochrome c ₃ was purified from extracts of Desulfobulbus elongatus. Redox potentiometry studies indicated that one of the four hemes has a uniquely high E _m value (−30 mV), whereas the other three have a lower redox potential with E _m values of −165 mV. In the presence of a membrane preparation from Db. elongatus the cytochrome c ₃ is partially reduced (23% at pH 7.6) by succinate. The physiological significance of the high redox potential of this cytochrome c ₃ is discussed as a function of the propionate metabolism of Desulfobulbus which is known to proceed via the succinate pathway.

【 授权许可】

Unknown