A deazaadenosine‐insensitive methylation of phosphatidylethanolamine is involved in lipoprotein secretion

FEBS Letters

Vance, Dennis E.¹  Vance, Jean E.¹ 

[1] The Department of Biochemistry, Faculty of Medicine, University of British Columbia, Vancouver, BC V6T 1W5, Canada

关键词: Phosphatidylethanolamine methylation Lipoprotein secretion Phosphatidylserine 3-Deazaadenosine Neplanocin;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    DZA;    3-deazaadenosine;   

DOI  :  10.1016/0014-5793(86)80820-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have examined the effect of inhibitors of methylation of phosphatidylethanolamine on lipoprotein secretion from cultured rat hepatocytes. The incorporation of [1-³H]ethanolamine into phosphatidylcholine of hepatocytes and secreted lipoproteins was inhibited by greater than 90% by the methylation inhibitors 3-deazaadenosine and Neplanocin. In addition, these compounds strongly inhibited the incorporation of [3-³H]serine into the choline moiety of phosphatidylcholine of the hepatocytes, but had no effect on incorporation of [3-³H]serine into secreted phosphatidylcholine. The results suggest that a pool of phosphatidylcholine targeted for lipoprotein secretion originates from phosphatidylethanolamine made from serine and this methylation reaction has the unique property of being insensitive to 3-deazaadenosine.

【 授权许可】

Unknown