ATP‐induced calcium mobilization and inositol 1,4,5‐trisphosphate formation in H‐35 hepatoma cells

FEBS Letters

Tennes, Karin A.¹  Putney, James W.¹  Horstman, Debra A.¹ 

[1] Division of Cellular Pharmacology, Medical College of Virginia, VCU, Richmond, VA 23298, USA

关键词: (H-35 cell) ATP receptor Ca2+-mobilizing receptor Purinergic receptor Inositol trisphosphate;   

DOI  :  10.1016/0014-5793(86)80809-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of ATP (but not epinephrine, angiotensin II, vasopressin, or platelet-activating factor) to H-35 hepatoma cells whose cellular lipids have been pre-labelled with [³H]inositol, causes a rapid increase in [³H]inositol trisphosphate. In H-35 cells pre-incubated in the presence of ⁴⁵Ca²⁺, ATP causes a similarly rapid release of ⁴⁵Ca²⁺. The concentration-effect relationships for inositol trisphosphate formation and Ca²⁺ efflux are similar to those reported previously for differentiated hepatocytes. These results demonstrate that at least one of the Ca²⁺-mobilizing receptors normally found on hepatocytes is functionally retained in the H-35 hepatoma cell line and thus could provide a useful model for the study of these receptor mechanisms in liver.

【 授权许可】

Unknown