Identification of Chl‐binding proteins in a PS II preparation from spinach

FEBS Letters

Vater, Joachim¹  Irrgang, Klaus-Dieter²  Renger, Gernot² 

[1] Institut für Biochemie und Molekular-biologie, Technische Universität Berlin, 1000 Berlin 12, Germany;Max-Volmer-Institut für Biophysikalische und Physikalische Chemie, Technische Universität Berlin, Germany

关键词: Chlorophyll-binding protein;    Photosystem II;    Solubilization;    (Spinach);    Chl;    chlorophyll;    PS;    photosystem;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(86)81389-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a mild solubilization procedure we have been able to detect several Chl-binding proteins by SDS-urea-PAGE. Their molecular masses were 46, 42, 34, 32, 29-24 and approx. 110 kDa (presumably a CPI contamination). The stability of the Chl-protein complexes depended strongly on experimental conditions. A very faint band at 34-30 kDa was resolved in visible light (in 40–50% of the experiments). With a more sensitive fluorometric technique (illumination at 366 nm, fluorescence detection in the red) we identified two Chlcontaining bands in the 30 kDa region. Limited proteolysis studies with trypsin revealed that the extent of the enzymatic degradation of some Chl-binding proteins in vitro is obviously dependent on pH and the presence of Ca²⁺.

【 授权许可】

Unknown