Regions of SV40 large T antigen necessary for oligomerization and complex formation with the cellular oncoprotein p53

FEBS Letters

Müller, Dorothee¹  Henning, Roland¹  Montenarh, Mathias¹  Vesco, Cesare²  Kemmerling, Gudrun¹ 

[1] Department of Biochemistry, University of Ulm, PO Box 4066, D-7900 Ulm, FRG;Institute of Cell Biology, Via G. Romagnosi, 00196 Rome, Italy

关键词: SV40 mutant;    SV40 large T antigen;    Oligomerization;    T antigen-p53 complex;    Functional region;   

DOI  :  10.1016/0014-5793(86)81386-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The simian virus 40 (SV40) T antigen is composed of 708 amino acids and forms monomers and various oligomers and, in small amounts, heterologous complexes with the cellular oncoprotein p53 (T-p53). Using SV40 mutants coding for T antigen fragments which are either deleted in the N-terminal half or truncated by various lengths at the C-terminal end, we found that a region between amino acids 114 and 152 and a C-terminal region up to amino acid 669 are essential for the formation of high M_r oligomers of T antigen. Furthermore, only the C-terminal end up to amino acid 669 is essential for T-p53 complex formation but not the N-terminus up to amino acid 152.

【 授权许可】

Unknown