Tumour promotor 12‐O‐tetradecanoylphorbol 13‐acetate inhibits angiotensin II‐induced inositol phosphate production and cytosolic Ca2+ rise in rat renal mesangial cells

FEBS Letters

Pfeilschifter, Josef¹ 

[1] Physiologisches Institut der Universität Zürich, Winterthwerstr. 190, CH-8057 Zürich, Switzerland

关键词: Angiotensin II;    Inositol trisphosphate;    Ca2+;    Phorbol tester;    Protein kinase C;    TPA;    12-O-tetradecanoylphorbol 13-acetate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    IP3;    myo-inositol trisphosphate;    IP2;    myo-inositol bisphosphate;    IP1;    myo-inositol monophosphate;    DG;    1;    2-diacylglycerol;    quin 2/AM;    2-[(2-bis(carboxymethyl)amino-5-methylphenoxy)methyl]-6-methoxy-8-bis-(carboxymethyl)aminoquinoline tetrakis(acetoxymethyl)ester;   

DOI  :  10.1016/0014-5793(86)80755-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Preincubation of rat renal mesangial cells with 12-O-tetradecanoylphorbol 13-acetate (TPA) strongly inhibited the increases of inositol phosphates and of free cytosolic Ca²⁺ induced by angiotensin II (10⁻⁷ M). TPA had no significant effect on the basal values of inositol phosphates and of free cytosolic Ca²⁺. Inhibition appeared already after 1 min and was maximal after 5 min. These effects occur without significant changes on angiotensin II binding in intact cells. The concentration of TPA needed (10⁻⁹–10⁻⁷ M) was in the range believed to cause specifically an activation of protein kinase C. Furthermore the biologically inactive phorbol ester 4α-phorbol 12,13-didecanoate was without effect. From the entirety of these results it is likely that protein kinase C inhibits angiotensin II activation of phospholipase C at a stage distal to receptor occupancy.

【 授权许可】

Unknown