Isolation and properties of the signal region from ovalbumin

FEBS Letters

Robinson, A.¹  Meredith, C.¹  Austen, B.M.¹ 

[1] Department of Surgery, St. George's Hospital Medical School, Cranmer Terrace, London SW17 ORE, England

关键词: Signal sequence;    Ovalbumin;    Translation;    Protein translocation;    Signal receptor;   

DOI  :  10.1016/0014-5793(86)80751-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A tryptic fragment (residues 21–47) containing the signal peptide of ovalbumin has been isolated by reversephase chromatography. The peptide is more active at inhibiting the processing of pre-prolactin in an in vitro translation system than fragments of ovalbumin isolated previously, and is similar in properties to synthetic signal peptides. The ovalbumin signal fragment is shown to bind to a protein component of salt-stripped pancreatic microsomal membranes, which is cross-linked under UV irradiation by a radioactive synthetic photoaffinity signal peptide probe to yield a radiolabelled 45 kDa protein.

【 授权许可】

Unknown