Flash‐induced absorption changes in photosystem I at low temperature: evidence that the electron acceptor A1 is vitamin K1

FEBS Letters

Sétif, P.¹  Brettel, K.¹  Mathis, P.¹ 

[1] Service de Biophysique, Département de Biologie, CEN de Saclay, 91191 Gif-sur-Yvette Cedex, France

关键词: Photosystem I;    Electron transfer;    Vitamin K1;    Absorption difference spectrum;    Low temperature Photosynthesis;   

DOI  :  10.1016/0014-5793(86)80746-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Low temperature flash absorption spectroscopy has been applied to elucidate the chemical nature of the secondary electron acceptor A₁ of photosystem I (PS-I). The flash-induced absorption changes measured in digitonin-fractionated spinach PS-I particles at 10 K between 240 and 525 nm are shown to comprise a major decay phase with t ~ 150 μs which has been attributed to the recombination reaction P-700⁺·A₁ → P-700·A₁ [(1984) Biochim. Biophys. Acta 767, 404-414]. We present the absorption difference spectrum of this reaction and demonstrate that it contains contributions in the ultraviolet due to A₁, which are characteristic of vitamin K₁ (phylloquinone).

【 授权许可】

Unknown