FEBS Letters | |
Flash‐induced absorption changes in photosystem I at low temperature: evidence that the electron acceptor A1 is vitamin K1 | |
Sétif, P.1  Brettel, K.1  Mathis, P.1  | |
[1] Service de Biophysique, Département de Biologie, CEN de Saclay, 91191 Gif-sur-Yvette Cedex, France | |
关键词: Photosystem I; Electron transfer; Vitamin K1; Absorption difference spectrum; Low temperature Photosynthesis; | |
DOI : 10.1016/0014-5793(86)80746-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Low temperature flash absorption spectroscopy has been applied to elucidate the chemical nature of the secondary electron acceptor A1 of photosystem I (PS-I). The flash-induced absorption changes measured in digitonin-fractionated spinach PS-I particles at 10 K between 240 and 525 nm are shown to comprise a major decay phase with t ~ 150 μs which has been attributed to the recombination reaction P-700+·A1 → P-700·A1 [(1984) Biochim. Biophys. Acta 767, 404-414]. We present the absorption difference spectrum of this reaction and demonstrate that it contains contributions in the ultraviolet due to A1, which are characteristic of vitamin K1 (phylloquinone).
【 授权许可】
Unknown
【 预 览 】
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