Putative ammo‐terminal presequence for β‐subunit of plant mitochondrial F1ATPase deduced from the amino‐terminal sequence of the mature subunit

FEBS Letters

Nakamura, Kenzo²  Sasaki, Takuji¹  Kobayashi, Kazuto²  Asahi, Tadashi²  Iwasaki, Yukimoto² 

[1] Laboratory of Physical Chemistry, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464, Japan;Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464, Japan

关键词: (Plant);    Mitochondria;    Organelle biogenesis;    F1ATPase;    β-Subunit;    Nuclear-encoded subunit;    Presequence;    DEAE;    diethylaminoethyl;   

DOI  :  10.1016/0014-5793(86)80731-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The α- and β-subunits of sweet potato mitochondrial F₁ATPase were purified from the F₁ complex by gel filtration and ion-exchange high-performance liquid chromatography. Isoelectric focussing and N-terminal amino acid sequencing indicated that the purified β-subunit contains at least two polypeptides similar to each other. The N-terminal 18 amino acid sequence of the β-subunit showed homology to the amino acid sequence of the tobacco mitochondrial F₁ATPase β-subunit precursor deduced from the nucleotide sequence [(1985) EMBO J. 4, 2159-2165] between residues 56 and 73, suggesting that the N-terminal 55 amino acids of the tobacco precursor constitute the presequence required for mitochondrial targetting.

【 授权许可】

Unknown