Accumulation and subcellular localization of glutelin‐2 transcripts during maturation of maize endosperm

FEBS Letters

Cortadas, Jordi¹  Pérez-Grau, Lluis¹  Puigdomènech, Pere¹  Palau, Jaume¹ 

[1] Institut de Biologia de Barcelona del CSIC, Jorge Girona Salgado 18-26, 08034 Barcelona, Spain

关键词: RNA (Maize endosperm) Glutelin Northern blot analysis Protein body Cereal storage protein;   

DOI  :  10.1016/0014-5793(86)80666-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of glutelin-2 genes has been studied during maize endosperm maturation and compared to the expression of two different zein genes. Glutelin-2 mRNA in endosperm accumulates in parallel to zein mRNAs, nevertheless, the opaque-2 mutation does not affect glutelin-2 transcription. Subcellular fractionation of endosperm tissue shows that glutelin-2 mRNA is accumulated in the protein body fraction indicating that these organelles are the place of translation of glutelin-2 mRNA. The results indicate that the levels of glutelin-2 transcripts in maize endosperm increase following a temporal pattern similar to that displayed by zein transcripts, the typical maize storage protein.

【 授权许可】

Unknown