Effects of some substrate analogs on aerobactin synthetase from Aerobacter aerogenes 62‐1

FEBS Letters

Viswanatha, Thammaiah¹  Appanna, Vasu D.¹ 

[1] Guelph Waterloo Centre for Graduate Work in Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada

关键词: Aerobactin synthetase Substrate analog Citric acid N6-Acetyl-N6-hydroxylysine Catalytic site;   

DOI  :  10.1016/0014-5793(86)80658-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tricarballylic acid was the only analog of citric acid that appeared to be a substrate for aerobactin synthetase. The others, succinic acid, glutaric acid and 3-methyl-3-hydroxyglutaric acid, though potent inhibitors of aerobactin synthesis, were neither activated nor utilised by the enzyme system. Aerobactin synthetase seemed to mediate the condensation of either N ⁶-n-butyryl-N ⁶-hydroxylysine, or N ⁶-succinyl-N ⁶-hydroxylysine, or N ⁶-acetyllysine and citric acid, in a manner analogous to that of the natural substrate, N ⁶-acetyl-N ⁶-hydroxylysine.

【 授权许可】

Unknown