FEBS Letters | |
Predicted sequence of the host‐protective immunogen of infectious bursal disease virus | |
McKern, Neil M.1  Hudson, Peter J.1  Fahey, Kevin J.2  Azad, Ahmed A.1  | |
[1] Division of Protein Chemistry, CSIRO, Parkville, Victoria 3052, Australia;Division of Animal Health, CSIRO, Parkville, Victoria 3052, Australia | |
关键词: Nucleotide sequence; Primary structure; Infectious bursal disease virus; Vaccine; viral RNA; Mab; monoclonal antibody; IBDV; infectious bursal disease virus; NBRF; the protein sequence database of the National Biomedical Research Foundation; Washington; USA; GENBANK; the nucleotide sequence database of the Los Alamos National Laboratory; Los Alamos; USA; | |
DOI : 10.1016/0014-5793(86)80587-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The genome of Australian strain 002-73 of infectious bursal disease virus (IBDV) has been cloned as cDNA fragments into an expression library based on pUR plasmid vectors. Recombinant colonies were selected with a monoclonal antibody specific for the 32-kDa host-protective immunogen of IBDV and fully characterized by nucleotide sequence analysis. The amino acid sequence of several tryptic peptides derived from the native 32-kDa structural protein has confirmed the coding region assignment and nucleotide sequence data. We believe this to be the first published sequence of a birnavirus-encoded protein and these data may provide the basis for an effective subunit vaccine against IBDV
【 授权许可】
Unknown
【 预 览 】
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RO201912020288051ZK.pdf | 334KB | download |