Predicted sequence of the host‐protective immunogen of infectious bursal disease virus

FEBS Letters

McKern, Neil M.¹  Hudson, Peter J.¹  Fahey, Kevin J.²  Azad, Ahmed A.¹ 

[1]Division of Protein Chemistry, CSIRO, Parkville, Victoria 3052, Australia

[2]Division of Animal Health, CSIRO, Parkville, Victoria 3052, Australia

关键词: Nucleotide sequence;    Primary structure;    Infectious bursal disease virus;    Vaccine;    viral RNA;    Mab;    monoclonal antibody;    IBDV;    infectious bursal disease virus;    NBRF;    the protein sequence database of the National Biomedical Research Foundation;    Washington;    USA;    GENBANK;    the nucleotide sequence database of the Los Alamos National Laboratory;    Los Alamos;    USA;   

DOI  :  10.1016/0014-5793(86)80587-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The genome of Australian strain 002-73 of infectious bursal disease virus (IBDV) has been cloned as cDNA fragments into an expression library based on pUR plasmid vectors. Recombinant colonies were selected with a monoclonal antibody specific for the 32-kDa host-protective immunogen of IBDV and fully characterized by nucleotide sequence analysis. The amino acid sequence of several tryptic peptides derived from the native 32-kDa structural protein has confirmed the coding region assignment and nucleotide sequence data. We believe this to be the first published sequence of a birnavirus-encoded protein and these data may provide the basis for an effective subunit vaccine against IBDV

【 授权许可】

Unknown