Adenosine transporters in chromaffin cells

FEBS Letters

Molina, P.¹  Torres, M.²  Miras-Portugal, M.T.² 

[1] Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Murcia, Murcia, Spain;Departamento de Bioquímica, Facultad de Biología , Murcia, Spain

关键词: Adenosine transport;    Chromaffin cell;    Dipyridamol;   

DOI  :  10.1016/0014-5793(86)80583-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chromaffin cells from bovine adrenal medulla are a useful model to approach adenosine transport and metabolism in neural cells. Dipyridamol has been shown to be an adenosine transport inhibitor with high affinity. To quantify the adenosine transporters a labelled dipyridamol analogue, [¹⁴C]dipyridamol acetate, was synthesized. This compound had a K _i = 5.3 ± 0.43 nM according to the Dixon method, and 4.58 ± 0.46 nM when the receptor number molarity was taken into account showing, like dipyridamol, a non-competitive mechanism. The high-affinity receptors present in chromaffin cells showed a K _d = 6.8 ± 0.8 nM and the receptor number was 630000 ± 40000 per cell.

【 授权许可】

Unknown