Na+‐coupled D‐glucose uptake and membrane order of enterocyte brush border membrane vesicles, under the effect of a series of N‐phenylcarbamates

FEBS Letters

Deltour, P.¹  Cambon-Gros, C.²  Fernandez, Y.²  Mitjavila, S.²  Boigegrain, R.-A.² 

[1] INSERM SC-13, 38 rue des 36 Ponts, 31400 Toulouse, France;INSERM U-87, 2 rue François Magendie 31400 Toulouse, France

关键词: Glucose transport;    Membrane fluidity;    ESR N-Phenylcarbamate;    Hydrophobic effect;    (Rat intestinal brush border);   

DOI  :  10.1016/0014-5793(86)80582-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The importance of the hydrophobic effect of exogenous substances and of modifications of membrane order on D-glucose uptake are still poorly defined. Our results show that the concentrative Na⁺ -coupled D-glucose uptake of rat enterocyte brush border membrane vesicles is inhibited by N-phenylcarbamate derivatives. Unlike other series of lipophilic compounds inhibiting D-glucose transport, these N-phenylcarbamates increase the membrane order. However, since the concentrations required for membrane order increase are much greater than those active on D-glucose uptake, the effects on lipid order cannot be resposible for the inhibition of D-glucose uptake. Measurements of D-glucose uptake under conditions of Na⁺ equilibrium show that these carbamates do not act directly on the carrier but indirectly by favouring the dissipation of the Na⁺ gradient.

【 授权许可】

Unknown