期刊论文详细信息
FEBS Letters | |
Physico‐chemical study of complex formation of DNA with wild‐type and mutant E. coli RNA polymerases Recognition properties of β‐subunit | |
Ozoline, O.N.1  Kamzolova, S.G.1  | |
[1] Institute of Biological Physics, Academy of Sciences of the USSR, Pushchino, Moscow Region, 142292, USSR | |
关键词: RNA polymerase Subunit structure Promoter recognition; | |
DOI : 10.1016/0014-5793(86)81155-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Complex formation of T7 DNA with RNA polymerase from E. coli B/r WU-36-10-11-12 (E. coli W 12) and its rifampicin-resistant mutant rpoB409 was studied. The rpoB409 mutant possesses a highly pleiotropic effect due to alteration in the RNA polymerase β-subunit structure. The two RNA polymerases have been previously shown to differ in gene selection during RNA synthesis on T7 DNA. In this study it was found that the change in selective properties of the mutant RNA polymerase occurs during its interaction with DNA, the general ability of the enzyme to melt DNA being unaffected.
【 授权许可】
Unknown
【 预 览 】
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RO201912020288007ZK.pdf | 579KB | download |