The effect of iron and agar on production of hydrogen peroxide by stimulated and activated mouse peritoneal macrophages

FEBS Letters

Brock, J.H.¹  Stevenson, Joan¹  Thompson, H.Lorraine¹ 

[1] University Department of Bacteriology and Immunology, Western Infirmary, Glasgow G11 6NT, Scotland

关键词: Macrophage Macrophage activation Hydrogen peroxide Iron Agar;   

DOI  :  10.1016/0014-5793(86)81153-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of iron on H₂O₂ production by mouse peritoneal macrophages exposed to opsonised zymosan has been investigated. Macrophages elicited with thioglycollate broth produced less H₂O₂ than macrophages activated by Corynebacterium parvum, and levels were not affected by prior incubation of the cells with 0.1 mM iron nitrilotriacetate. However, preincubation with the iron chelator desferrioxamine (1 mM) reduced H₂O₂ production by both types of macrophages. Incubation of macrophages with agar, a component of thioglycollate broth, also reduced H₂O₂ production, particularly by C. parvum-activated macrophages. The results indicate that although iron appears to be necessary for H₂O₂ production by macrophages, the low level of production by thioglycollate-elicited macrophages is not due to an inadequate level of metabolically utilisable iron, but may be a result of prior ingestion of agar present in the broth.

【 授权许可】

Unknown