Transition kinetics of the conversion of blue to purple bacteriorhodopsin upon magnesium binding

FEBS Letters

Tsuji, Kinko¹  Hess, Benno¹  Zubov, Boris¹ 

[1] Max-Planck-Institut für Ernährungsphysiologie, D-4600 Dortmund, FRG

关键词: Blue bacteriorhodopsin Purple bacteriorhodopsin Magnesium binding Stopped-flow method Kinetic amplitude spectrum Rate constant;   

DOI  :  10.1016/0014-5793(86)80543-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Magnesium binding to cation#x2010;depleted blue bacteriorhodopsin (b-bR) was studied spectrophotometrically as well as by following stopped-flow kinetics. There exist three kinetically different steps in the binding process, yielding purple bacteriorhodopsin (p-bR). Since only the firtst step is dependent on the concentration of the reactants, the reaction scheme can be proposed as the simplest model, with MgbR being the first intermediate and ΣI denoting a set of successive intermediates. According to this model k ₁, k ₋₁ and k ₂ are calculated to be 2.8 × 10⁴ M⁻¹ · s⁻¹, 5.0 × 10 s⁻¹ and 1 × 10⁻² s⁻¹, respectively.

【 授权许可】

Unknown