Specificity studies on anti‐histone H1 antibodies obtained by different immunization methods

FEBS Letters

Zlatanova, Jordanka¹  Srebreva, Ljuba¹ 

[1] Institute of Molecular Biology and Institute of Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

关键词: Histone H1 Histone antibody Antibody specificity ELISA;    PBS;    phosphate-buffered saline;    MCF;    microcomplement fixation;    ELISA;    enzyme-linked immunosorbent assay;    HMG;    high mobility group (proteins);   

DOI  :  10.1016/0014-5793(86)80523-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies were elicited against chromatographically purified histone H l subfractions or against their complexes with RNA and their specificity studied by enzyme-linked immunosorbent assay. The results show that complexing of the pure protein with RNA (i) does not lead to any significant increase in the antibody titer and (ii) results in obtaining antibodies predominantly against the common antigenic determinants present in the HI histone class. On the other hand, using pure histone fractions for immunization gives rise to antibody populations reacting mainly with the subfraction-specific determinants on the histone molecule. In view of these results the literature data should be interpreted with caution.

【 授权许可】

Unknown