Polyadenylation site heterogeneity in mRNA encoding the precursor of the barley toxin β‐hordothionin

FEBS Letters

Paz-Ares, Javier¹  Royo, Joaquin¹  García-Olmedo, Francisco¹  Carbonero, Pilar¹  Ponz, Fernando¹  Hernández-Lucas, Carlos¹ 

[1] Departamento de Bioquímica, ETS Ingenieros Agrónomos-UPM, 28040 Madrid, Spain

关键词: (Barley endosperm) Plant toxin β-Hordothionin Cleavage/poly(A) site cDNA sequence;   

DOI  :  10.1016/0014-5793(86)80519-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two cDNA clones, pTH2 and pTH3, encoding the precursor of the barley toxin β-hordothionin have been identified and their nucleotide sequences determined. These sequences are identical, except that pTH2 is 22 bp longer than pTH3 at the 5'-end and that the cleavage/poly(A) site of the mRNA represented by pTH3 is 4 positions further downstream from the single 5'-AAUAAA-3' polyadenylation signal than that of the pTH2 mRNA. In contrast, the cleavage/poly(A) site of the α-hordothionin mRNA is 30 positions downstream from a second polyadenylation signal. The deduced amino acid sequence of the β-hordothionin precursor differs from that of α-hordothionin at 13 out of 127 positions.

【 授权许可】

Unknown