The structure of C hordein

FEBS Letters

Field, J.M.²  Shewry, P.R.¹  Baker, A.M.¹  Tatham, A.S.¹ 

[1] Rothamsted Experimental Station, Harpenden AL5 2JQ, England;The Miln Marsters Group Ltd, Plant Breeding and Research Centre, Docking, Norfolk, England

关键词: Hordein Molecular dimensions Secondary structure β-turn;   

DOI  :  10.1016/0014-5793(86)80514-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intrinsic viscosity of C hordein was determined in 70% () aqueous ethanol (± 10 mM NaCl) and 0.1 M acetic acid at temperatures between 15.3 and 50°C. Calculations based on the assumption (supported by previously published results) that C hordein has an ordered conformation indicated that the molecule is rod shaped, with molecular dimensions varying from about 360 × 17 Å to 265 × 20 Å. Circular dichroism spectroscopy indicated a secondary structure rich in β-turns, with some conformational differences in the two solvents and at different temperatures. The results are compatible with C hordein having a helical secondary structure based on repetitive β-turns.

【 授权许可】

Unknown