Effect of ionic strength on production of cAMP‐ and Ca2+ ‐independent protein kinase from rat liver plasma membrane

FEBS Letters

Sakanoue, Youichirou¹  Yamamura, Hirohei¹  Hashimoto, Eikichi¹  Mizuta, Keiko² 

[1]Department of Biochemistry, Fukui Medical School, Matsuoka, Fukui 910-11, Japan

[2]Center of Medical Technology, Fukui Medical School, Matsuoka, Fiikui 910-11, Japan

关键词: Protein kinase C Limited proteolysis Membrane-bound enzyme Ionic strength Na+ influx Growth factor;   

DOI  :  10.1016/0014-5793(86)80511-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Production of cAMP- and Ca²⁺-independent protein kinase was stimulated when rat liver plasma membrane was incubated with increasing concentrations of NaCl. This protein kinase release was diminished by addition of protease inhibitor. The molecular mass of this enzyme was approx. 50 kDa and a high concentration of Mg²⁺ was required for whole histone phosphorylation. These properties are similar to those of the protease-activated form of protein kinase C. The NaCl effect could be replaced by other salts such as LiCl and NaHCO₃. These results suggest that membrane-bound protein kinase C is activated by limited proteolysis corresponding to an increase in ionic strength.

【 授权许可】

Unknown