Primary structure of a major sialyl‐saccharide alditol from human amniotic mucins expressing the tumor‐associated sialyl‐X antigenic determinant

FEBS Letters

Egge, Heinz²  Hanisch, Franz-Georg¹  Uhlenbruck, Gerhard¹  Peter-Katalinic, Jasna² 

[1] Institut für Physiologische Chemie der Universität Bonn, Nußallee 11, D-5300 Bonn 1, FRG;Abteilung Immunbiologie, 1. Medizinische Universitätsklinik, Kerpener Straße 15, D-5000 Köln 41 FRG

关键词: Tumor-associated antigen Sialyl-X determinant Carbohydrate structure Mass spectrometry Mucin (Human amniotic fluid);   

DOI  :  10.1016/0014-5793(86)80507-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A major sialyl-saccharide alditol isolated from mucins of human amniotic fluid was purified by high-performance liquid chromatography followed by high-performance thin-layer chromatography of the methylated derivative and subjected to structural analyses which comprised fast atom bombardment and electron impact-mass spectrometry, methylation analysis, exoglycosidase digestion and CrO₃ oxidation. The structure of a disialylated pentasaccharide characterized by a sialyl-X antigenic determinant was established:

【 授权许可】

Unknown